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KMID : 1094720080130040424
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Biotechnology and Bioprocess Engineering
2008 Volume.13 No. 4 p.424 ~ p.430
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Effects of culture media on hCTLA4Ig production and protein expression patterns in transgenic rice cell suspension cultures
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Yu Da-Hyun
Song Mi-Na
Lim Jung-Ae
Kim Dong-Il
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Abstract
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The effects of culture media on the production of human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig) and intracellular protein expression patterns were investigated in transgenic rice cell suspension cultures. Using comparative proteomic analysis, changes in the intracellular proteome in different culture media were identified. Culture media were found to be an important factor for the production of the recombinant target protein in this expression system, which was under the control of the rice α-amylase 3D (RAmy3D) promoter. In terms of hCTLA4Ig production, the N6 medium produced a 3.7-fold higher level of protein than the AA medium. In addition, the N6 medium provided better protein stability and cell viability. In the intracellular proteome analysis, we identified eight proteomes that were differentially expressed. These results could provide valuable information for the improvement of cell growth and target protein production.
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KEYWORD
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rice cell suspension cultures, hCTLA4Ig, proteomics, AA medium, N6 medium
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